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We know that water content of LBM is 70 ml/100 g.Assume all this water is in LBM & that fat is water free.What is his lean body mass (LBM)? What is his fat mass? In a healthy adult male weighing 70 kg, total body water (TBW) was measured to be 42 L.Water content of LBM is constant - 70 ml /100 g tissue.Note: the water content of LBM is constant.Total body mass = fat mass + fat free mass.Principle: same – indicator dilution principle.Measure the fraction of RBC tagged with Cr 51.Inject a known amount of Cr 51 labeled RBC intravenously.Use Chromium 51 labeled RBC as the indicator.Measuring red cell volumeNote: measuring is different from calculating something In a healthy 70 kg adult male, plasma volume (measured using the Evans Blue dye) was found to be 3000 ml.Blood volume = plasma volume × 100 / (100 – Hct) Image from īlood volume = plasma volume × (100/100-Hct).Volume of cells: packed cell volume (PCV).What is the relationship between blood volume and plasma volume? In health, the total volume of transcellular fluids is ICF volume = Total body water – ECF volume.ISF volume = ECF volume – plasma volume.Cannot be measured it is calculated (estimated).How would you measure interstitial fluid (ISF) volume? ![]() Thus, volume of distribution (Vd) of sucrose = A / C = 95 mg / 0.01 mg/ml = 9500 ml = 9.5 L (= ECF volume). ![]()
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